Search results for "MitoTracker Green"

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A method for measuring mitochondrial mass and activity

2007

Mitochondria, responsible for the energy-generating process essential for the cell metabolism, differ for the number, localization and activity in animal cells and tissues in relation to the energetic needs. Using fluorescent probes specific for mitochondria, Mitotracker Green (MTG) and Orange (MTO), and Confocal Laser-Scanning Microscope (CLSM), we elaborated a method to measure in vivo the mitochondrial mass and activity, in sea urchin Paracentrotus lividus eggs and embryos. The analysis of captured images, revealed a variation of mitochondrial distribution and an increase of activity after fertilization.

ConfocalClinical BiochemistryBiomedical Engineeringsea urchin.BioengineeringMitochondrionmitochondrial activityParacentrotus lividusMitoTracker GreenHuman fertilizationIn vivobiology.animalSettore BIO/06 - Anatomia Comparata E CitologiaSea urchinmitochondrial mabiologyBrief ReportEmbryoCell BiologyAnatomybiology.organism_classificationFluorescenceCell biologyMitoTracker OrangeCLSM; mitochondrial mass; mitochondrial activity; MitoTracker Green; MitoTracker Orange; sea urchin.CLSMBiotechnologyCytotechnology

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